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The main limitation of the study is the variable delays between injuries and blood sampling. This may have affected biomarkers with a short half-life in blood, such as H-FABP, IL-10, and S100B. Furthermore, for NF-L, the sampling time-points might have been too close to the injury (64). Earlier mean sampling time would probably have resulted in different sensitivities and specificities for the panels.

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• 47
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In addition, we could not use the levels of UCH-L1 from the Human Neurology 4-Plex assay in the current analyses because the coefficients of variation were at a level where the results are not reliable. We also used the National Institute for Health and Care Excellence criteria for head CT imaging, and the results might not be applicable for other head CT rules due to differences in case selection. The fairly small study cohort also increases the risk of over-fitting bias, and therefore, the results should be verified and validated in a larger cohort.

• ECG signals were also recorded in the CHB‐MIT Scalp EEG, TUSZ, Helsinki University Hospital, and Siena Scalp EEG datasets.
• Noncontinuous datasets such as the University of Bonn and Kaggle UPenn datasets only include ictal segments without preictal segments, so they should only be used for seizure detection classification.
• ,
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• Further, iEEG recordings are able to capture more epileptiform discharges compared with scalp recordings due to high signal attenuation by the skull from the source towards the scalp.
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Primary characteristics and structural properties of publicly available datasets. Primary characteristics of a dataset can be subdivided into 7 features (left), while structural properties consist of 3 features (right). The strengths of this study are the use of several biomarkers of different cellular origins in the same cohort, the use of sensitive advanced analytics, and a prospectively recruited well-characterized study population. Although a minority of the screened patients were included in the current analyses, the patient selection did not introduce a significant bias, as the only difference was sex distribution.

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• This can be seen in Table 8, where the Helsinki University Hospital EEG dataset has a median epoch length of 74 minutes, and the Neurovista ictal dataset has different seizure segment lengths, with an average of 107 seconds.

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There were patients with missing data on pupil reactivity, events of hypoxia, hypotension, and hypoglycemia, and these were excluded from the comparative analysis. Data on GCS, ISS, (27), and HCTS sum are presented in medians and ranges. Differences between groups are analyzed with the Mann–Whitney U-test. The levels of the biomarkers were not normally distributed and are presented as medians with interquartile ranges (IQRs). Differences in biomarker levels between the two outcome groups were analyzed with the Mann–Whitney U-test. Aviator online casino game broke into the industry not long ago, but managed to make thousands, if not millions, of fans across the world.

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, 12 However, this is not common practice, which impacts the reproducibility and generalizability of these findings. 46

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• The studies show that single biomarkers tend to have low specificities when sensitivity is set above 90%.
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• One method to overcome this particular issue is in order to select datasets that will have an identical electrode placement along with a quantity of channels in this article the International 10–20 EEG placement.
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Table 2

Number of channels, placement method of the channels, and type of signal for the datasets. The datasets generated for this study are available on request to the corresponding author. First, familiarize yourself with the rules and pay table for this game before starting to play. If you want to try out Spribe Gaming’s Aviator for free, you can also play a demo instead of the full version. This will allow you to get used to the game and learn how it works without risking any actual money. With multiple deposit and withdrawal methods available, you can easily manage your account anywhere.

Moreover, the assays utilized in this study are developed for research purposes, limiting the generalizability of the results. However, this limitation also concerns most of the current TBI biomarker studies because there is a paucity of commercialized assays for clinical TBI diagnostics. The possibility of some degree of selection bias should be noted, as only a third of the patients treated at the recruiting hospital were eventually enrolled in the study. The current cohort is somewhat less severely injured than those in which the HCTS has been earlier validated. The HCTS was originally designed using a neurocritical care cohort.

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• As these biomarkers are of different cellular origins, we planned to investigate each separately as well as combined.
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As discussed earlier, iEEG signals have a higher signal‐to‐noise ratio compared with surface EEG as they are less affected by artifacts and environmental factors. 1 , 29 Further, iEEG recordings are able to capture more epileptiform discharges compared with scalp recordings due to high signal attenuation by the skull from the source towards the scalp.